How cytometer can Save You Time, Stress, and Money.

How cytometer can Save You Time, Stress, and Money.

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Data-enriched characterization of cell populations. Morphological data from photographs can include into the richness of flow cytometry details. One example is, the determine reveals an or else regular apoptosis assay applying Annexin V and PI, introducing mobile imaging to characterize cells in Every single inhabitants to reveal morphologically distinctive capabilities. These insights could not have already been acquired from multiplex staining alone.

As samples are obtained to the Attune CytPix Flow Cytometer, the large-velocity brightfield digital camera captures and merchants pictures of detected situations in a rate around 6,000 illustrations or photos per second, depending on the flow charge and picture size. For bigger overall flexibility, Attune Cytometric Computer software lets you alter the graphic capture frequency as essential and choose a selected gate for picture capture, if needed.

Blend acoustic concentrating know-how with higher-parameter Evaluation for adaptable spectral unmixing and traditional workflows.

Find out new insights into your experimental knowledge by visualizing populations utilizing combined fluorescence and morphology information. Our application interface wizards permit downsampling, concatenation (merged information) and dimensionality equipment that are designed to the computer software for an intuitive, streamlined data Investigation practical experience.

*Pricing is indicative only and is subject to change. Make sure you Speak to your Agilent agent with any queries. The data set forth herein is not a suggestion or even a determination, the two of that happen to be subject to credit history acceptance, terms and conditions, and availability with Agilent. Agilent Monetary Answers is out there in selected nations.

Right here, a highly trained user has gated singlets confidently. After analyzing the manual singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate is made up of more than four% aggregates.

Optimizing gating technique. Even robust handbook singlet gating is error-susceptible and continues to be a subjective choice level in almost all flow cytometry assays. Imaging can be used to verify and adjust gates to incorporate only single cells of desire.

Steady picture high-quality even at higher throughput Acoustic focusing in addition to a large-speed digital camera combine to image these T cells continually at small or high flow charges. Conveniently change emphasis and digital camera configurations to meet experimental prerequisites.

The Attune NxT cytometer is the dependable workhorse instrument your lab can depend on for steady and reliable general performance. The Attune CytPix design features a large-speed brightfield camera and AI-pushed impression Examination application, with recent updates which includes equipment Studying instruments and an intuitive user interface, enabling Increased knowledge precision, accuracy, and a lot quicker insights into sample biology.

Consumers can check out function images in actual-time to verify sample excellent, optimize protocols, and history the morphology of each and every mobile population identified for Examination. Imaging seize also Rewards from acoustic focusing, which really helps to position the cells so that a sharp, centered graphic is obtained.

AI-driven automated image Examination Together with the Attune Cytometric Software package interprets party attributes into distinctive morphology parameters which might be combined with standard fluorescence and scatter parameters to expose hugely reproducible population improvements and special subsets.

We previously shown the power of imaging CAR-T/Ramos cell interactions. Let’s check out just the populace of best fascination, the double favourable occasions, to learn more. We can easily now use extended morphological parameters (below, circularity and skewness of depth) to even more look at the attributes of those populations and refine gating on these functions, escalating knowledge robustness.

Leverage the power of Sophisticated technology and innovation to accelerate your discoveries. Belief from the Attune Flow Cytometer family members to provide the trustworthiness, speed, and precision that set your get the job done apart.

No wash/no lyse leukocyte Examination with imaging. Cells had been acquired from 24-hour-old blood by dilution in 1 mM EDTA ( Professional UX/UI enables users to teach AI models for expanded applications. (A) The “Prepare Product” element supplies annotation equipment and authentic-time model education accuracy.

Gating on these prolonged parameters allows you to speedily and accurately determine populations of fascination and ensure gating method with little if any guide evaluation. The Attune software offers access to condition-of-the-art equipment Finding out Examination tools inside of an expertly intended consumer interface, which makes it exceptionally simple to operate.

The Attune Xenith cytometer is actually a higher-parameter spectral analyzer, giving both equally spectral unmixing and standard flow capabilities for complete and flexible cell Evaluation.

Agilent Value Assure assures you at the very least ten years of instrument use through the date you buy. Otherwise, we will credit rating you with the residual value of the check here system toward an upgraded product.

Below, we exhibit that by gating techniques applying picture-dependent quantitative parameters can distinguish interacting cells from coincident gatherings to extra precisely review interacting cells.